Reducing the effects of PCR amplification and sequencing artifacts on 16S rRNA-based studies.

The advent of next generation sequencing has coincided with a growth in interest in using these approaches to better understand the role of the structure and function of the microbial communities in human, animal, and environmental health. Yet, use of next generation sequencing to perform 16S rRNA g...

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Bibliographic Details
Main Authors: Patrick D Schloss, Dirk Gevers, Sarah L Westcott
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3237409?pdf=render