SMOOT libraries and phage-induced directed evolution of Cas9 to engineer reduced off-target activity.

SMOOT libraries and phage-induced directed evolution of Cas9 to engineer reduced off-target activity.

RNA-guided endonucleases such as Cas9 provide efficient on-target genome editing in cells but may also cleave at off-target loci throughout the genome. Engineered variants of Streptococcus pyogenes Cas9 (SpCas9) have been developed to globally reduce off-target activity, but individual off-targets m...

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Bibliographic Details
Main Authors: Derek Cerchione, Katherine Loveluck, Eric L Tillotson, Fred Harbinski, Jen DaSilva, Chase P Kelley, Elise Keston-Smith, Cecilia A Fernandez, Vic E Myer, Hariharan Jayaram, Barrett E Steinberg
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0231716

Internet

https://doi.org/10.1371/journal.pone.0231716

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