A Systematic Comparison of Purification and Normalization Protocols for Quantitative MicroRNA Expressional Profiling in Insulin-Producing Cells

A Systematic Comparison of Purification and Normalization Protocols for Quantitative MicroRNA Expressional Profiling in Insulin-Producing Cells

As microRNAs (miRs) are gaining increasing attention as key regulators of cellular processes, expressional quantification is widely applied. However, in the processing of relatively quantified data, the importance of testing the stability of several reference mRNAs and/or miRs and choosing among the...

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Bibliographic Details
Main Authors: Anna Lindeløv Vestergaard, Maaike Blankestijn, Jonathan Lucien Stahl, Emil Marek Heymans Pallesen, Claus Heiner Bang-Berthelsen, Flemming Pociot, Guy Wayne Novotny, Morten Lundh, Thomas Mandrup-Poulsen
Format: Article
Language:English
Published: MDPI AG 2016-06-01
Series:International Journal of Molecular Sciences
Subjects:
method
microRNA
purification
qPCR
quantification
normalization
pancreas
Online Access:http://www.mdpi.com/1422-0067/17/6/896

Internet

http://www.mdpi.com/1422-0067/17/6/896

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