Effect of divalent metal ions on the activity and stability of β-galactosidase isolated from Kluyveromyces lactis

Effect of divalent metal ions on the activity and stability of β-galactosidase isolated from Kluyveromyces lactis

In this study, it was demonstrated that β-galactosidase can be deactivated and reactivated with EDTA and divalent metal ions. The enzyme was deactivated after 20 minutes in EDTA solution. Maximal deactivation at the lowest EDTA concentration (10-3 mol.L-1) occurred in the presence of Tris-HCl buffer...

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Bibliographic Details
Main Authors: Paulo Roberto Adalberto, Antonio Carlos Massabni, Eleonora Cano Carmona, Antônio José Goulart, Daniela Parreira Marques, Rubens Monti
Format: Article
Language:English
Published: São Paulo State University (UNESP) 2010-12-01
Series:Revista de Ciências Farmacêuticas Básica e Aplicada
Subjects:
β-galactosidase, divalent metallic ions, enzyme activity, stability
Online Access:http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/1037

Internet

http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/1037

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