Tannase enhances the anti-inflammatory effect of grape pomace in Caco-2 cells treated with IL-1β
Grape pomace (GP) is rich in polymeric polyphenolics and glycosides which have lower bioefficacy than monomeric and aglycone counterparts. The aim of this study was to determine whether tannin acyl hydrolase [tannase (TNS)] can improve the antioxidant and anti-inflammatory actions of GP in Caco-2 ce...
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doaj-2fc0a662fa3644388228f27d586134d22021-04-30T07:09:16ZengElsevierJournal of Functional Foods1756-46462017-02-01296976Tannase enhances the anti-inflammatory effect of grape pomace in Caco-2 cells treated with IL-1βIsabela M. Martins0Gabriela A. Macedo1Juliana A. Macedo2Bruna S. Roberto3Qianru Chen4Jeffrey B. Blumberg5Chung-Yen Oliver Chen6Bioprocesses Laboratory, Faculty of Food Engineering, University of Campinas, C.P.: 6121, CEP 13083-862 Campinas, SP, Brazil; Antioxidants Research Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USABioprocesses Laboratory, Faculty of Food Engineering, University of Campinas, C.P.: 6121, CEP 13083-862 Campinas, SP, BrazilBioprocesses Laboratory, Faculty of Food Engineering, University of Campinas, C.P.: 6121, CEP 13083-862 Campinas, SP, BrazilBioprocesses Laboratory, Faculty of Food Engineering, University of Campinas, C.P.: 6121, CEP 13083-862 Campinas, SP, BrazilAntioxidants Research Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USAAntioxidants Research Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USAAntioxidants Research Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA; Corresponding author at: Antioxidants Research Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, 711 Washington Street, Boston, MA 02111, USA.Grape pomace (GP) is rich in polymeric polyphenolics and glycosides which have lower bioefficacy than monomeric and aglycone counterparts. The aim of this study was to determine whether tannin acyl hydrolase [tannase (TNS)] can improve the antioxidant and anti-inflammatory actions of GP in Caco-2 cells treated with IL-1β. TNS increased quercetin content by 45% and decreased quercetin-3-O-rutinoside by 71% as compared to untreated GP. Further, TNS increased total phenols and DPPH, ORAC, and FRAP values by 39, 57, 215, and 12%, respectively. However, GP and GPTNS at 100 and 200 μg/mL (dry extract wt/v) displayed comparable efficacy in the reduction of ROS in Caco-2 cells. After 24 h pre-treatment, GPTNS (200 μg/mL) decreased IL-1β-induced PGE2 and IL-8 secretion by 107 and 83% respectively, and down-regulated NF-κB activation by 63%. Thus, TNS appears to enhance antioxidant and anti-inflammatory activities of GP polyphenolics and suggests their use as a value-added agroindustrial residue.http://www.sciencedirect.com/science/article/pii/S1756464616304005Grape pomaceTannasePhenolic compoundsCaco-2 cellsGut inflammation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Isabela M. Martins Gabriela A. Macedo Juliana A. Macedo Bruna S. Roberto Qianru Chen Jeffrey B. Blumberg Chung-Yen Oliver Chen |
spellingShingle |
Isabela M. Martins Gabriela A. Macedo Juliana A. Macedo Bruna S. Roberto Qianru Chen Jeffrey B. Blumberg Chung-Yen Oliver Chen Tannase enhances the anti-inflammatory effect of grape pomace in Caco-2 cells treated with IL-1β Journal of Functional Foods Grape pomace Tannase Phenolic compounds Caco-2 cells Gut inflammation |
author_facet |
Isabela M. Martins Gabriela A. Macedo Juliana A. Macedo Bruna S. Roberto Qianru Chen Jeffrey B. Blumberg Chung-Yen Oliver Chen |
author_sort |
Isabela M. Martins |
title |
Tannase enhances the anti-inflammatory effect of grape pomace in Caco-2 cells treated with IL-1β |
title_short |
Tannase enhances the anti-inflammatory effect of grape pomace in Caco-2 cells treated with IL-1β |
title_full |
Tannase enhances the anti-inflammatory effect of grape pomace in Caco-2 cells treated with IL-1β |
title_fullStr |
Tannase enhances the anti-inflammatory effect of grape pomace in Caco-2 cells treated with IL-1β |
title_full_unstemmed |
Tannase enhances the anti-inflammatory effect of grape pomace in Caco-2 cells treated with IL-1β |
title_sort |
tannase enhances the anti-inflammatory effect of grape pomace in caco-2 cells treated with il-1β |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2017-02-01 |
description |
Grape pomace (GP) is rich in polymeric polyphenolics and glycosides which have lower bioefficacy than monomeric and aglycone counterparts. The aim of this study was to determine whether tannin acyl hydrolase [tannase (TNS)] can improve the antioxidant and anti-inflammatory actions of GP in Caco-2 cells treated with IL-1β. TNS increased quercetin content by 45% and decreased quercetin-3-O-rutinoside by 71% as compared to untreated GP. Further, TNS increased total phenols and DPPH, ORAC, and FRAP values by 39, 57, 215, and 12%, respectively. However, GP and GPTNS at 100 and 200 μg/mL (dry extract wt/v) displayed comparable efficacy in the reduction of ROS in Caco-2 cells. After 24 h pre-treatment, GPTNS (200 μg/mL) decreased IL-1β-induced PGE2 and IL-8 secretion by 107 and 83% respectively, and down-regulated NF-κB activation by 63%. Thus, TNS appears to enhance antioxidant and anti-inflammatory activities of GP polyphenolics and suggests their use as a value-added agroindustrial residue. |
topic |
Grape pomace Tannase Phenolic compounds Caco-2 cells Gut inflammation |
url |
http://www.sciencedirect.com/science/article/pii/S1756464616304005 |
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