Diagnosis of Barmah Forest virus infection by a nested real-time SYBR green RT-PCR assay.

Barmah Forest virus (BFV) is a mosquito borne (+) ssRNA alphavirus found only in Australia. It causes rash, myalgia and arthralgia in humans and is usually diagnosed serologically. We developed a real-time PCR assay to detect BFV in an effort to improve diagnosis early in the course of infection. Th...

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Bibliographic Details
Main Authors: Linda Hueston, Cheryl S Toi, Neisha Jeoffreys, Tania Sorrell, Gwendolyn Gilbert
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3720699?pdf=render