CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine <i>miR-17-92</i> Cluster

CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine <i>miR-17-92</i> Cluster

Successful RNAi applications depend on strategies allowing stable and persistent expression of minimal gene silencing triggers without perturbing endogenous gene expression. In this study, we proposed an endogenous microRNA (miRNA) cluster as a novel integration site for small hairpin RNAs (shRNAs)....

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Bibliographic Details
Main Authors: Chao Lu, Daxin Pang, Mengjing Li, Hongming Yuan, Tingting Yu, Peixuan Huang, Jianing Li, Xue Chen, Huping Jiao, Zicong Xie, Hongsheng Ouyang
Format: Article
Language:English
Published: MDPI AG 2019-02-01
Series:Cells
Subjects:
RNAi
shRNA
miRNA-17-92 cluster
CRISPR/Cas9
classical swine fever virus
Online Access:https://www.mdpi.com/2073-4409/8/2/113

Internet

https://www.mdpi.com/2073-4409/8/2/113

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