CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine <i>miR-17-92</i> Cluster
Successful RNAi applications depend on strategies allowing stable and persistent expression of minimal gene silencing triggers without perturbing endogenous gene expression. In this study, we proposed an endogenous microRNA (miRNA) cluster as a novel integration site for small hairpin RNAs (shRNAs)....
Main Authors: | Chao Lu, Daxin Pang, Mengjing Li, Hongming Yuan, Tingting Yu, Peixuan Huang, Jianing Li, Xue Chen, Huping Jiao, Zicong Xie, Hongsheng Ouyang |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2019-02-01
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Series: | Cells |
Subjects: | |
Online Access: | https://www.mdpi.com/2073-4409/8/2/113 |
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