Validation of a Combined Transcriptome and T Cell Receptor Alpha/Beta (TRA/TRB) Repertoire Assay at the Single Cell Level for Paucicellular Samples

Validation of a Combined Transcriptome and T Cell Receptor Alpha/Beta (TRA/TRB) Repertoire Assay at the Single Cell Level for Paucicellular Samples

Transcriptomics can be combined with TRA and TRB clonotype analysis at the single cell level. The aim of this study was to validate this approach on the ICELL8 Single-Cell system and to evaluate its usefulness to analyse clinical paucicellular samples. For this purpose, we carefully selected T cell...

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Bibliographic Details
Main Authors: Nicolle H. R. Litjens, Anton W. Langerak, Amy C. J. van der List, Mariska Klepper, Maaike de Bie, Zakia Azmani, Alexander T. den Dekker, Rutger W. W. Brouwer, Michiel G. H. Betjes, Wilfred F. J. Van IJcken
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-08-01
Series:Frontiers in Immunology
Subjects:
single cell transcriptomics
single T cell receptor repertoire analysis
cDNA
combined transcriptome and T cell receptor repertoire assay
low cell number
Online Access:https://www.frontiersin.org/article/10.3389/fimmu.2020.01999/full

Internet

https://www.frontiersin.org/article/10.3389/fimmu.2020.01999/full

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