Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT

<p>Abstract</p> <p>Background</p> <p>Cryopreservation of PBMC and/or overnight shipping of samples are required for many clinical trials, despite their potentially adverse effects upon immune monitoring assays such as MHC-peptide tetramer staining, cytokine flow cytomet...

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Main Authors: Morse Michael A, Clay Timothy M, Summers Amanda, Chang Jennie C, Payne Janice K, Kuus-Reichel Kristine, Ghanekar Smita A, Maino Vernon C, Bhatia Sonny, Moon James, Maecker Holden T, Lyerly H Kim, DeLaRosa Corazon, Ankerst Donna P, Disis Mary L
Format: Article
Language:English
Published: BMC 2005-07-01
Series:BMC Immunology
Online Access:http://www.biomedcentral.com/1471-2172/6/17