Enhanced solubility and antimicrobial activity of alamethicin in aqueous solution by complexation with γ-cyclodextrin
Alamethicin (ALM) inhibits the growth of bacteria by disrupting their cell membrane. ALM exhibits poor solubility in aqueous medium thus limiting its application. A method is proposed for encapsulation of ALM with γ-cyclodextrin (γ-CD) by forming a complex of higher solubility with thermal and pH st...
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doaj-311ab4596350480ab9320e74502cc3492021-04-30T07:12:33ZengElsevierJournal of Functional Foods1756-46462018-01-0140700706Enhanced solubility and antimicrobial activity of alamethicin in aqueous solution by complexation with γ-cyclodextrinMeng-Ke Zhang0Yuan Lyu1Xiao Zhu2Jin-Peng Wang3Zheng-Yu Jin4Ganesan Narsimhan5School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, ChinaDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, United StatesResearch Computing, Rosen Center for Advanced Computing, Purdue University, West Lafayette, IN 47907, United StatesSchool of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Corresponding authors.Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, United States; Corresponding authors.Alamethicin (ALM) inhibits the growth of bacteria by disrupting their cell membrane. ALM exhibits poor solubility in aqueous medium thus limiting its application. A method is proposed for encapsulation of ALM with γ-cyclodextrin (γ-CD) by forming a complex of higher solubility with thermal and pH stability as confirmed by FT-IR and DSC, thus greatly improving its application in food safety. Molecular dynamics simulation suggested a stable configuration of the complex when hydrophobic residues are inserted into the hydrophobic cavity of γ-CD. Aggregation of the complex was inhibited at higher γ-CD/ALM molar ratio. γ-CD/ALM complex exhibited significant antimicrobial activity against L. monocytogenes with the minimum inhibitory concentration being dependent on γ-CD/ALM molar ratio (>0.429 mg/mL for 1:1; >1.094 mg/mL for 5:1; >2.078 mg/mL for 10:1). Optimum γ-CD/ALM molar ratio for maximum antimicrobial activity is the result of competing effects of higher solubility and shielding of hydrophobic ALM groups by γ-CD.http://www.sciencedirect.com/science/article/pii/S1756464617307442AlamethicinCyclodextrinAntimicrobial peptideL. monocytogenesComplexMolecular dynamics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Meng-Ke Zhang Yuan Lyu Xiao Zhu Jin-Peng Wang Zheng-Yu Jin Ganesan Narsimhan |
spellingShingle |
Meng-Ke Zhang Yuan Lyu Xiao Zhu Jin-Peng Wang Zheng-Yu Jin Ganesan Narsimhan Enhanced solubility and antimicrobial activity of alamethicin in aqueous solution by complexation with γ-cyclodextrin Journal of Functional Foods Alamethicin Cyclodextrin Antimicrobial peptide L. monocytogenes Complex Molecular dynamics |
author_facet |
Meng-Ke Zhang Yuan Lyu Xiao Zhu Jin-Peng Wang Zheng-Yu Jin Ganesan Narsimhan |
author_sort |
Meng-Ke Zhang |
title |
Enhanced solubility and antimicrobial activity of alamethicin in aqueous solution by complexation with γ-cyclodextrin |
title_short |
Enhanced solubility and antimicrobial activity of alamethicin in aqueous solution by complexation with γ-cyclodextrin |
title_full |
Enhanced solubility and antimicrobial activity of alamethicin in aqueous solution by complexation with γ-cyclodextrin |
title_fullStr |
Enhanced solubility and antimicrobial activity of alamethicin in aqueous solution by complexation with γ-cyclodextrin |
title_full_unstemmed |
Enhanced solubility and antimicrobial activity of alamethicin in aqueous solution by complexation with γ-cyclodextrin |
title_sort |
enhanced solubility and antimicrobial activity of alamethicin in aqueous solution by complexation with γ-cyclodextrin |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2018-01-01 |
description |
Alamethicin (ALM) inhibits the growth of bacteria by disrupting their cell membrane. ALM exhibits poor solubility in aqueous medium thus limiting its application. A method is proposed for encapsulation of ALM with γ-cyclodextrin (γ-CD) by forming a complex of higher solubility with thermal and pH stability as confirmed by FT-IR and DSC, thus greatly improving its application in food safety. Molecular dynamics simulation suggested a stable configuration of the complex when hydrophobic residues are inserted into the hydrophobic cavity of γ-CD. Aggregation of the complex was inhibited at higher γ-CD/ALM molar ratio. γ-CD/ALM complex exhibited significant antimicrobial activity against L. monocytogenes with the minimum inhibitory concentration being dependent on γ-CD/ALM molar ratio (>0.429 mg/mL for 1:1; >1.094 mg/mL for 5:1; >2.078 mg/mL for 10:1). Optimum γ-CD/ALM molar ratio for maximum antimicrobial activity is the result of competing effects of higher solubility and shielding of hydrophobic ALM groups by γ-CD. |
topic |
Alamethicin Cyclodextrin Antimicrobial peptide L. monocytogenes Complex Molecular dynamics |
url |
http://www.sciencedirect.com/science/article/pii/S1756464617307442 |
work_keys_str_mv |
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