Purification and functional analysis of the recombinant protein isolated from E. coli by employing three different methods of bacterial lysis

Purification and functional analysis of the recombinant protein isolated from E. coli by employing three different methods of bacterial lysis

In this paper, the purification of the human recombinant protein expressed in E. coli using the GSTGene Fusion System, by applying various methods of bacterial lysis: sonication, freeze/thaw and beadbeating, is presented. The study was an attempt to compare the properties of the proteins obtained by...

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Bibliographic Details
Main Authors: Mojsin Marija, Nikčević Gordana T., Kovačević-Grujičić Nataša R., Savić Tijana, Petrović Isidora, Stevanović Milena
Format: Article
Language:English
Published: Serbian Chemical Society 2005-01-01
Series:Journal of the Serbian Chemical Society
Subjects:
protein purification
bacterial lysis
protein yield
protein-dna interaction
Online Access:http://www.doiserbia.nb.rs/img/doi/0352-5139/2005/0352-51390507943M.pdf

Internet

http://www.doiserbia.nb.rs/img/doi/0352-5139/2005/0352-51390507943M.pdf

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