Detection and quantification of VEGFR-1 in the nuclei of tumor cells by a new flow cytometry-based method

Detection and quantification of VEGFR-1 in the nuclei of tumor cells by a new flow cytometry-based method

Flow cytometry using fluorescent antibodies (FC) is the method of choice for the quantitation of proteins expressed at the surface or inside the cell, but, however, does not allow to selectively measure nuclear expression. We therefore sought to develop a method for the extraction of intact cell nuc...

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Bibliographic Details
Main Authors: Ekaterina Tyrsina, Sergey Nikulitskiy, Andrey Inshakov, Oxana Ryabaya, Dmitry Tyrsin
Format: Article
Language:English
Published: Taylor & Francis Group 2019-01-01
Series:Journal of Immunotoxicology
Subjects:
nuclear extraction
flow cytometry
vascular endothelial growth factor receptor-type 1
a431 and bro tumor cell lines
phf postnatal human fibroblasts
Online Access:http://dx.doi.org/10.1080/1547691X.2019.1598522

Internet

http://dx.doi.org/10.1080/1547691X.2019.1598522

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