Optimisation of the Protocol for the LIVE/DEAD® BacLightTM Bacterial Viability Kit for Rapid Determination of Bacterial Load

Optimisation of the Protocol for the LIVE/DEAD® BacLightTM Bacterial Viability Kit for Rapid Determination of Bacterial Load

Rapid antimicrobial susceptibility testing is needed to reduce prescription of inappropriate antibiotics. A rapid alternative to standard culture-based testing is to determine reductions in cell viability using the LIVE/DEAD® BacLightTM Bacterial Viability Kit. We optimised the kit protocol for this...

Full description

Bibliographic Details
Main Authors: Julia Robertson, Cushla McGoverin, Frédérique Vanholsbeeck, Simon Swift
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-04-01
Series:Frontiers in Microbiology
Subjects:
viability
fluorescence
Escherichia coli
SYTO 9
propidium iodide
crosstalk
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.00801/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2019.00801/full

Similar Items