The Glucocorticoid Receptor NR3C1 in Testicular Peritubular Cells is Developmentally Regulated and Linked to the Smooth Muscle-Like Cellular Phenotype
Whether glucocorticoids (GC) can directly affect human testicular functions is not well understood. A predominant site of GC receptor (GR; <i>NR3C1</i>) expression in the adult testis are peritubular smooth muscle-like cells, which express smooth muscle actin (ACTA2), contract and thereb...
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doaj-3149a2655d404bd590e8163c113fd30e2020-11-25T02:41:32ZengMDPI AGJournal of Clinical Medicine2077-03832020-03-01996196110.3390/jcm9040961The Glucocorticoid Receptor NR3C1 in Testicular Peritubular Cells is Developmentally Regulated and Linked to the Smooth Muscle-Like Cellular PhenotypeHarald Welter0Carola Herrmann1Nils Dellweg2Annika Missel3Christiane Thanisch4Henryk F. Urbanski5Frank-Michael Köhn6J. Ullrich Schwarzer7Annette Müller-Taubenberger8Artur Mayerhofer9Biomedical Center, Cell Biology, Anatomy III, Ludwig Maximilian University of Munich, 82152 Planegg-Martinsried, GermanyBiomedical Center, Cell Biology, Anatomy III, Ludwig Maximilian University of Munich, 82152 Planegg-Martinsried, GermanyBiomedical Center, Cell Biology, Anatomy III, Ludwig Maximilian University of Munich, 82152 Planegg-Martinsried, GermanyBiomedical Center, Cell Biology, Anatomy III, Ludwig Maximilian University of Munich, 82152 Planegg-Martinsried, GermanyIbidi GmbH, 82166 Gräfelfing, GermanyDivision of Neuroscience, Oregon National Primate Research Center, Beaverton, OR 97006-248, USAAndrologicum, 80331 Munich, GermanyAndrology Center, 81241 Munich, GermanyBiomedical Center, Cell Biology, Anatomy III, Ludwig Maximilian University of Munich, 82152 Planegg-Martinsried, GermanyBiomedical Center, Cell Biology, Anatomy III, Ludwig Maximilian University of Munich, 82152 Planegg-Martinsried, GermanyWhether glucocorticoids (GC) can directly affect human testicular functions is not well understood. A predominant site of GC receptor (GR; <i>NR3C1</i>) expression in the adult testis are peritubular smooth muscle-like cells, which express smooth muscle actin (ACTA2), contract and thereby are involved in sperm transport. In contrast to the adult, neither GR nor ACTA2, or elastin (ELN) were detected in the peritubular compartment before puberty in non-human primate testes. In isolated human testicular peritubular cells (HTPCs), activation of GR by dexamethasone (Dex) caused the translocation of GR to the nucleus and stimulated expression of <i>ACTA2</i> and <i>ELN</i>, without affecting the expression of collagens. Cytoskeletal ACTA2-rearrangements were observed and were associated with an increased ability to contract. Our results indicate post-pubertal testicular roles of GC in the maintenance of the contractile, smooth muscle-like phenotype of peritubular cells.https://www.mdpi.com/2077-0383/9/4/961human male fertilityhuman testisdexamethasoneelastinglucocorticoidcytoskeleton |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Harald Welter Carola Herrmann Nils Dellweg Annika Missel Christiane Thanisch Henryk F. Urbanski Frank-Michael Köhn J. Ullrich Schwarzer Annette Müller-Taubenberger Artur Mayerhofer |
spellingShingle |
Harald Welter Carola Herrmann Nils Dellweg Annika Missel Christiane Thanisch Henryk F. Urbanski Frank-Michael Köhn J. Ullrich Schwarzer Annette Müller-Taubenberger Artur Mayerhofer The Glucocorticoid Receptor NR3C1 in Testicular Peritubular Cells is Developmentally Regulated and Linked to the Smooth Muscle-Like Cellular Phenotype Journal of Clinical Medicine human male fertility human testis dexamethasone elastin glucocorticoid cytoskeleton |
author_facet |
Harald Welter Carola Herrmann Nils Dellweg Annika Missel Christiane Thanisch Henryk F. Urbanski Frank-Michael Köhn J. Ullrich Schwarzer Annette Müller-Taubenberger Artur Mayerhofer |
author_sort |
Harald Welter |
title |
The Glucocorticoid Receptor NR3C1 in Testicular Peritubular Cells is Developmentally Regulated and Linked to the Smooth Muscle-Like Cellular Phenotype |
title_short |
The Glucocorticoid Receptor NR3C1 in Testicular Peritubular Cells is Developmentally Regulated and Linked to the Smooth Muscle-Like Cellular Phenotype |
title_full |
The Glucocorticoid Receptor NR3C1 in Testicular Peritubular Cells is Developmentally Regulated and Linked to the Smooth Muscle-Like Cellular Phenotype |
title_fullStr |
The Glucocorticoid Receptor NR3C1 in Testicular Peritubular Cells is Developmentally Regulated and Linked to the Smooth Muscle-Like Cellular Phenotype |
title_full_unstemmed |
The Glucocorticoid Receptor NR3C1 in Testicular Peritubular Cells is Developmentally Regulated and Linked to the Smooth Muscle-Like Cellular Phenotype |
title_sort |
glucocorticoid receptor nr3c1 in testicular peritubular cells is developmentally regulated and linked to the smooth muscle-like cellular phenotype |
publisher |
MDPI AG |
series |
Journal of Clinical Medicine |
issn |
2077-0383 |
publishDate |
2020-03-01 |
description |
Whether glucocorticoids (GC) can directly affect human testicular functions is not well understood. A predominant site of GC receptor (GR; <i>NR3C1</i>) expression in the adult testis are peritubular smooth muscle-like cells, which express smooth muscle actin (ACTA2), contract and thereby are involved in sperm transport. In contrast to the adult, neither GR nor ACTA2, or elastin (ELN) were detected in the peritubular compartment before puberty in non-human primate testes. In isolated human testicular peritubular cells (HTPCs), activation of GR by dexamethasone (Dex) caused the translocation of GR to the nucleus and stimulated expression of <i>ACTA2</i> and <i>ELN</i>, without affecting the expression of collagens. Cytoskeletal ACTA2-rearrangements were observed and were associated with an increased ability to contract. Our results indicate post-pubertal testicular roles of GC in the maintenance of the contractile, smooth muscle-like phenotype of peritubular cells. |
topic |
human male fertility human testis dexamethasone elastin glucocorticoid cytoskeleton |
url |
https://www.mdpi.com/2077-0383/9/4/961 |
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