Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay

Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay

The present study compared two heating methods currently used for antigen retrieval (AR) immunostaining: the microwave oven and the steam cooker. Myosin-V, a molecular motor involved in vesicle transport, was used as a neuronal marker in honeybee Apis mellifera brains fixed in formalin. Overall, the...

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Bibliographic Details
Main Authors: LUCIANA KAREN CALÁBRIA, RENATA ROLAND TEIXEIRA, SYBELLI MAGDA COELHO GONÇALVES, ANDREIA BARCELOS PASSOS LIMA, ANA ALICE DINIZ DOS SANTOS, ANTÔNIO ROBERTO MARTINS, FOUED SALMEN ESPINDOLA
Format: Article
Language:English
Published: BMC 2010-01-01
Series:Biological Research
Subjects:
antigen retrieval
microwave oven
steam cooker
Online Access:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100002

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http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100002

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