Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay
The present study compared two heating methods currently used for antigen retrieval (AR) immunostaining: the microwave oven and the steam cooker. Myosin-V, a molecular motor involved in vesicle transport, was used as a neuronal marker in honeybee Apis mellifera brains fixed in formalin. Overall, the...
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doaj-314dc77f4023475b838854312c786cbc2020-11-24T21:12:47ZengBMCBiological Research0716-97600717-62872010-01-01431712Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assayLUCIANA KAREN CALÁBRIARENATA ROLAND TEIXEIRASYBELLI MAGDA COELHO GONÇALVESANDREIA BARCELOS PASSOS LIMAANA ALICE DINIZ DOS SANTOSANTÔNIO ROBERTO MARTINSFOUED SALMEN ESPINDOLAThe present study compared two heating methods currently used for antigen retrieval (AR) immunostaining: the microwave oven and the steam cooker. Myosin-V, a molecular motor involved in vesicle transport, was used as a neuronal marker in honeybee Apis mellifera brains fixed in formalin. Overall, the steam cooker showed the most satisfactory AR results. At 100 ºC, tissue morphology was maintained and revealed epitope recovery, while evaporation of the AR solution was markedly reduced; this is important for stabilizing the sodium citrate molarity of the AR buffer and reducing background effects. Standardization of heat-mediated AR of formalin-fixed and paraffin-embedded tissue sections results in more reliable immunostaining of the honeybee brain.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100002antigen retrievalmicrowave ovensteam cooker |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
LUCIANA KAREN CALÁBRIA RENATA ROLAND TEIXEIRA SYBELLI MAGDA COELHO GONÇALVES ANDREIA BARCELOS PASSOS LIMA ANA ALICE DINIZ DOS SANTOS ANTÔNIO ROBERTO MARTINS FOUED SALMEN ESPINDOLA |
spellingShingle |
LUCIANA KAREN CALÁBRIA RENATA ROLAND TEIXEIRA SYBELLI MAGDA COELHO GONÇALVES ANDREIA BARCELOS PASSOS LIMA ANA ALICE DINIZ DOS SANTOS ANTÔNIO ROBERTO MARTINS FOUED SALMEN ESPINDOLA Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay Biological Research antigen retrieval microwave oven steam cooker |
author_facet |
LUCIANA KAREN CALÁBRIA RENATA ROLAND TEIXEIRA SYBELLI MAGDA COELHO GONÇALVES ANDREIA BARCELOS PASSOS LIMA ANA ALICE DINIZ DOS SANTOS ANTÔNIO ROBERTO MARTINS FOUED SALMEN ESPINDOLA |
author_sort |
LUCIANA KAREN CALÁBRIA |
title |
Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay |
title_short |
Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay |
title_full |
Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay |
title_fullStr |
Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay |
title_full_unstemmed |
Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay |
title_sort |
comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee apis mellifera: myosin-v assay |
publisher |
BMC |
series |
Biological Research |
issn |
0716-9760 0717-6287 |
publishDate |
2010-01-01 |
description |
The present study compared two heating methods currently used for antigen retrieval (AR) immunostaining: the microwave oven and the steam cooker. Myosin-V, a molecular motor involved in vesicle transport, was used as a neuronal marker in honeybee Apis mellifera brains fixed in formalin. Overall, the steam cooker showed the most satisfactory AR results. At 100 ºC, tissue morphology was maintained and revealed epitope recovery, while evaporation of the AR solution was markedly reduced; this is important for stabilizing the sodium citrate molarity of the AR buffer and reducing background effects. Standardization of heat-mediated AR of formalin-fixed and paraffin-embedded tissue sections results in more reliable immunostaining of the honeybee brain. |
topic |
antigen retrieval microwave oven steam cooker |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100002 |
work_keys_str_mv |
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