Efficient isolation of Magnolia protoplasts and the application to subcellular localization of MdeHSF1

Efficient isolation of Magnolia protoplasts and the application to subcellular localization of MdeHSF1

Abstract Background Magnolia is a woody ornamental plant, which is widely used in urban landscaping. However, its lengthy juvenile period and recalcitrance to regeneration impedes functional characterization of its genes. Results We developed an efficient protoplast isolation and transient expressio...

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Bibliographic Details
Main Authors: Yamei Shen, Dong Meng, Kim McGrouther, Junhong Zhang, Lailiang Cheng
Format: Article
Language:English
Published: BMC 2017-05-01
Series:Plant Methods
Subjects:
Magnolia
Protoplast isolation
Heat shock transcription factor
Heat stress
Subcellular localization
Online Access:http://link.springer.com/article/10.1186/s13007-017-0193-3

Internet

http://link.springer.com/article/10.1186/s13007-017-0193-3

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