Role of NAD+-Dependent Malate Dehydrogenase in the Metabolism of Methylomicrobium alcaliphilum 20Z and Methylosinus trichosporium OB3b

Role of NAD+-Dependent Malate Dehydrogenase in the Metabolism of Methylomicrobium alcaliphilum 20Z and Methylosinus trichosporium OB3b

We have expressed the l-malate dehydrogenase (MDH) genes from aerobic methanotrophs Methylomicrobium alcaliphilum 20Z and Methylosinus trichosporium OB3b as his-tagged proteins in Escherichia coli. The substrate specificities, enzymatic kinetics and oligomeric states of the MDHs have been characteri...

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Bibliographic Details
Main Authors: Olga N. Rozova, Valentina N. Khmelenina, Ksenia A. Bocharova, Ildar I. Mustakhimov, Yuri A. Trotsenko
Format: Article
Language:English
Published: MDPI AG 2015-02-01
Series:Microorganisms
Subjects:
l-malate dehydrogenase
catalytic efficiency
thermostability
tricarboxylic acid cycle
methanotrophs
Methylomicrobium alcaliphilum
Methylosinus trichosporium
Online Access:http://www.mdpi.com/2076-2607/3/1/47

Internet

http://www.mdpi.com/2076-2607/3/1/47

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