Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer

Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer

Summary: Dendritic cell (DC) maturation induced by Toll-like receptor (TLR) agonists requires the activation of downstream metabolic changes. Here, we provide a detailed protocol to measure glycolysis, mitochondrial respiration, and fatty acid oxidation in mouse bone-marrow-derived DCs with the Seah...

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Bibliographic Details
Main Authors: Kazuhito Gotoh, Yurie Takata, Yuya Nakashima, Soichi Mizuguchi, Keishi Komori, Dongchon Kang
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Cell biology
Cell culture
Cell isolation
Cell-based assays
Immunology
Metabolism
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721001088

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http://www.sciencedirect.com/science/article/pii/S2666166721001088

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