Tuning Relative Polypeptide Expression to Optimize Assembly, Yield and Downstream Processing of Bispecific Antibodies

Tuning Relative Polypeptide Expression to Optimize Assembly, Yield and Downstream Processing of Bispecific Antibodies

Bispecific antibodies (bsAbs) are often composed of several polypeptide chains that have to be expressed adequately to enable optimal assembly and yield of the bsAb. κλ bodies are a bispecific format with a native IgG structure, composed of two different light chains that pair w...

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Bibliographic Details
Main Authors: Giovanni Magistrelli, Guillemette Pontini, Yves Poitevin, Pauline Malinge, Jérémie Bourguignon, Florence Gauye, Elise Fleury, Nicolas Plèche, Lydia Galissaires, Nicolas Fischer
Format: Article
Language:English
Published: MDPI AG 2018-08-01
Series:Antibodies
Subjects:
affinity chromatography
bispecific antibody
codon optimization
co-expression
transient transfection
stable pools
product related impurities
Online Access:http://www.mdpi.com/2073-4468/7/3/29

Internet

http://www.mdpi.com/2073-4468/7/3/29

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