Complementarity of Selective Culture and qPCR for Colistin Resistance Screening in Fresh and Frozen Pig Cecum Samples

Retrospective studies involving the screening of frozen stored collections of samples are commonplace when a new threat emerges, but it has been demonstrated that the freeze-thaw process can affect bacterial viability. The study of colistin-resistant bacteria in human and animal samples is an exampl...

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Bibliographic Details
Main Authors: Pedro Miguela-Villoldo, Miguel A. Moreno, Marta Hernández, David Rodríguez-Lázaro, Alejandro Gallardo, Carmen Borge, Alberto Quesada, Lucas Domínguez, María Ugarte-Ruiz
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-11-01
Series:Frontiers in Microbiology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2020.572712/full