Current Practice in Bicistronic IRES Reporter Use: A Systematic Review

Current Practice in Bicistronic IRES Reporter Use: A Systematic Review

Bicistronic reporter assays have been instrumental for transgene expression, understanding of internal ribosomal entry site (IRES) translation, and identification of novel cap-independent translational elements (CITE). We observed a large methodological variability in the use of bicistronic reporter...

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Bibliographic Details
Main Authors: Guus Gijsbertus Hubert van den Akker, Federico Zacchini, Bas Adrianus Catharina Housmans, Laura van der Vloet, Marjolein Maria Johanna Caron, Lorenzo Montanaro, Tim Johannes Maria Welting
Format: Article
Language:English
Published: MDPI AG 2021-05-01
Series:International Journal of Molecular Sciences
Subjects:
bicistronic reporter
dicistronic reporter
mRNA translation
ribosome
IRES
systematic review
Online Access:https://www.mdpi.com/1422-0067/22/10/5193

Internet

https://www.mdpi.com/1422-0067/22/10/5193

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