Rapid and efficient enhancer cloning and in vivo screening using the developing chick embryo

Rapid and efficient enhancer cloning and in vivo screening using the developing chick embryo

Summary: Here, we describe a highly efficient, medium-throughput strategy for cloning and in vivo screening of putative enhancers using the chick embryo. By incorporating 48 unique nanotags for use in NanoString nCounter® across three different fluorescent reporters and developing a rapid and effici...

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Bibliographic Details
Main Authors: Ruth M. Williams, Tatjana Sauka-Spengler
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Model Organisms
Molecular Biology
Gene Expression
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002148

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http://www.sciencedirect.com/science/article/pii/S2666166721002148

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