gRNA validation for wheat genome editing with the CRISPR-Cas9 system

gRNA validation for wheat genome editing with the CRISPR-Cas9 system

Abstract Background The CRISPR-Cas9 system is a powerful and versatile tool for crop genome editing. However, achieving highly efficient and specific editing in polyploid species can be a challenge. The efficiency and specificity of the CRISPR-Cas9 system depends critically on the gRNA used. Here, w...

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Bibliographic Details
Main Authors: Taj Arndell, Niharika Sharma, Peter Langridge, Ute Baumann, Nathan S. Watson-Haigh, Ryan Whitford
Format: Article
Language:English
Published: BMC 2019-10-01
Series:BMC Biotechnology
Subjects:
Genome editing
CRISPR-Cas9
gRNA
Wheat
Triticum aestivum
Protoplasts
Online Access:http://link.springer.com/article/10.1186/s12896-019-0565-z

Internet

http://link.springer.com/article/10.1186/s12896-019-0565-z

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