Analysis of Pseudomonas aeruginosa cell envelope proteome by capture of surface-exposed proteins on activated magnetic nanoparticles.

Analysis of Pseudomonas aeruginosa cell envelope proteome by capture of surface-exposed proteins on activated magnetic nanoparticles.

We report on specific magneto-capturing followed by Multidimensional Protein Identification Technology (MudPIT) for the analysis of surface-exposed proteins of intact cells of the bacterial opportunistic pathogen Pseudomonas aeruginosa. The magneto-separation of cell envelope fragments from the solu...

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Bibliographic Details
Main Authors: Davide Vecchietti, Dario Di Silvestre, Matteo Miriani, Francesco Bonomi, Mauro Marengo, Alessandra Bragonzi, Lara Cova, Eleonora Franceschi, Pierluigi Mauri, Giovanni Bertoni
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3511353?pdf=render

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http://europepmc.org/articles/PMC3511353?pdf=render

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