Single-molecule analysis reveals self assembly and nanoscale segregation of two distinct cavin subcomplexes on caveolae

Single-molecule analysis reveals self assembly and nanoscale segregation of two distinct cavin subcomplexes on caveolae

In mammalian cells three closely related cavin proteins cooperate with the scaffolding protein caveolin to form membrane invaginations known as caveolae. Here we have developed a novel single-molecule fluorescence approach to directly observe interactions and stoichiometries in protein complexes fro...

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Bibliographic Details
Main Authors: Yann Gambin, Nicholas Ariotti, Kerrie-Ann McMahon, Michele Bastiani, Emma Sierecki, Oleksiy Kovtun, Mark E Polinkovsky, Astrid Magenau, WooRam Jung, Satomi Okano, Yong Zhou, Natalya Leneva, Sergey Mureev, Wayne Johnston, Katharina Gaus, John F Hancock, Brett M Collins, Kirill Alexandrov, Robert G Parton
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2014-01-01
Series:eLife
Subjects:
caveolae
single-molecule
cell-free protein expression
Online Access:https://elifesciences.org/articles/01434

Internet

https://elifesciences.org/articles/01434

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