Detection of Mitochondrial Mutations Through Isothermal Nucleic Acid Amplification Coupled With Clustered Regularly Interspaced Short Palindromic Repeat-Associated Endonuclease Cas13a

Detection of Mitochondrial Mutations Through Isothermal Nucleic Acid Amplification Coupled With Clustered Regularly Interspaced Short Palindromic Repeat-Associated Endonuclease Cas13a

The clustered regularly interspaced short palindromic repeat (CRISPR)-associated endonuclease Cas13a can specifically bind and cleave RNA. After nucleic acid pre-amplification, bacterial Cas13a has been used to detect genetic mutations. In our study, using a transcription-mediated amplification toge...

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Bibliographic Details
Main Authors: Hua Jiang, Kun Duan, Xu Han, Jun Wang, Xiao Liu, Maoxiao Yan, Yunxiu Wang, Hongyan Liu, Huiling Shi, Xiaoqing Gao, Chuan Ouyang, Xue Fu, Xinxin Zhang, Chao Liu
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-01-01
Series:Frontiers in Genetics
Subjects:
isothermal reaction
transcription-mediated amplification
clustered regularly interspaced short palindromic repeat
mitochondrial mutation
nonsyndromic hearing loss
Online Access:https://www.frontiersin.org/articles/10.3389/fgene.2020.622671/full

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https://www.frontiersin.org/articles/10.3389/fgene.2020.622671/full

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