Optimized Method of Extracting Rice Chloroplast DNA for High-Quality Plastome Resequencing and de Novo Assembly

Optimized Method of Extracting Rice Chloroplast DNA for High-Quality Plastome Resequencing and de Novo Assembly

Chloroplasts, which perform photosynthesis, are one of the most important organelles in green plants and algae. Chloroplasts maintain an independent genome that includes important genes encoding their photosynthetic machinery and various housekeeping functions. Owing to its non-recombinant nature, l...

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Bibliographic Details
Main Authors: Takeshi Takamatsu, Marouane Baslam, Takuya Inomata, Kazusato Oikawa, Kimiko Itoh, Takayuki Ohnishi, Tetsu Kinoshita, Toshiaki Mitsui
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-02-01
Series:Frontiers in Plant Science
Subjects:
de novo assembly
chloroplast DNA
next-generation sequencing
plastid genome
NUPTs
MTPTs
Online Access:http://journal.frontiersin.org/article/10.3389/fpls.2018.00266/full

Internet

http://journal.frontiersin.org/article/10.3389/fpls.2018.00266/full

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