An automated workflow for quantifying RNA transcripts in individual cells in large data-sets

An automated workflow for quantifying RNA transcripts in individual cells in large data-sets

Advanced molecular probing techniques such as single molecule fluorescence in situ hybridization (smFISH) or RNAscope can be used to assess the quantity and spatial location of mRNA transcripts within cells. Quantifying mRNA expression in large image sets usually involves automated counting of fluor...

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Bibliographic Details
Main Authors: Matthew C. Pharris, Tzu-Ching Wu, Xinping Chen, Xu Wang, David M. Umulis, Vikki M. Weake, Tamara L. Kinzer-Ursem
Format: Article
Language:English
Published: Elsevier 2017-01-01
Series:MethodsX
Subjects:
Cell-by-cell relative integrated transcript (CCRIT) quantification
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016117300274

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http://www.sciencedirect.com/science/article/pii/S2215016117300274

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