Affinity chromatography with pseudobiospecific ligands on high-performance supports for purification of proteins of biotechnological interest

High-performance affinity matrices were obtained by attaching pseudobiospecific ligands to hollow-fibre membranes. The neutral protease contained in FlavourzymeTM was purified to homogeneity with Yellow 4R-HE affinity hollow-fibre membranes. Immobilisation of Red HE-3B allowed purification of a milk...

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Bibliographic Details
Main Authors: N.B. Iannucci, F.J. Wolman, S.A. Camperi, A.A.N. Cañizo, M. Grasselli, O. Cascone
Format: Article
Language:English
Published: Brazilian Society of Chemical Engineering 2003-03-01
Series:Brazilian Journal of Chemical Engineering
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322003000100006