Construction and expression of a synthetic gene encoding nonstructural glycoprotein NS1 of dengue 2 virus in Pichia pastoris

Construction and expression of a synthetic gene encoding nonstructural glycoprotein NS1 of dengue 2 virus in Pichia pastoris

Objectives: To express and characterize NS1 of Indonesian-specific DENV2 virus in Pichia pastoris (P. pastoris). Methods: A codon optimized synthetic gene derived from the DENV-2 NS1 amino acid sequences was synthesized commercially and inserted into the P. pastoris pPICZαA expression vector. The re...

Full description

Bibliographic Details
Main Authors: Fernita Puspasari, Riski Dwimalida Putri, Aisyah, Raden Roro Rika Damayanti, Anita Yuwita, Bachti Alisjahbana, Sukwan Handali, Ihsanawati, Dessy Natalia
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2017-08-01
Series:Asian Pacific Journal of Tropical Biomedicine
Subjects:
DENV 2
Dengue virus
NS1 protein
Diagnostic kit
Pichia pastoris
Online Access:http://www.sciencedirect.com/science/article/pii/S2221169117307839

Internet

http://www.sciencedirect.com/science/article/pii/S2221169117307839

Similar Items