New Glutamine-Containing Substrates for the Assay of Cysteine Peptidases From the C1 Papain Family

New Glutamine-Containing Substrates for the Assay of Cysteine Peptidases From the C1 Papain Family

New substrates with glutamine in the P1-position are introduced for the assay of peptidases from the C1 papain family, with a general formula of Glp-Phe-Gln-X, where Glp is pyroglutamyl and X is pNA (p-nitroanilide) or AMC (4-amino-7-methylcoumaride). The substrates have a simple structure, and C1 c...

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Bibliographic Details
Main Authors: Irina Y. Filippova, Elena A. Dvoryakova, Nikolay I. Sokolenko, Tatiana R. Simonyan, Valeriia F. Tereshchenkova, Nikita I. Zhiganov, Yakov E. Dunaevsky, Mikhail A. Belozersky, Brenda Oppert, Elena N. Elpidina
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-10-01
Series:Frontiers in Molecular Biosciences
Subjects:
cathepsin B
cathepsin L
multicomponent enzyme mixtures
selective peptide substrates
Tenebrio molitor
Tribolium castaneum
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2020.578758/full

Internet

https://www.frontiersin.org/articles/10.3389/fmolb.2020.578758/full

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