Optimization of five qPCR protocols toward the detection and the quantification of antimicrobial resistance genes in environmental samples

Here, we describe the optimization and validation of five quantitative PCR (qPCR) assays by employing the SYBRGreen chemistry paired with melting curve analysis to detect and quantify clinically relevant antimicrobial resistance genes (ARGs) (i.e. ermB, blaCTXM1-like, blaCMY-2, qnrA and qnrS) from e...

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Bibliographic Details
Main Authors: Roberta Tolosi, Lisa Carraro, Andrea Laconi, Alessandra Piccirillo
Format: Article
Language:English
Published: Elsevier 2021-01-01
Series:MethodsX
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016121002818