An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells.

An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells.

A set of mutated Encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) elements with varying strengths is generated by mutating the translation initiation codons of 10(th), 11(th), and 12(th) AUG to non-AUG triplets. They are able to control the relative expression of multiple genes...

Full description

Bibliographic Details
Main Authors: Esther Y C Koh, Steven C L Ho, Mariati, Zhiwei Song, Xuezhi Bi, Muriel Bardor, Yuansheng Yang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3857217?pdf=render

Internet

http://europepmc.org/articles/PMC3857217?pdf=render

Similar Items