Development and Optimization of a Germination Assay and Long-Term Storage for <i>Cannabis sativa</i> Pollen

Development and Optimization of a Germination Assay and Long-Term Storage for <i>Cannabis sativa</i> Pollen

Pollen viability and storage is of great interest to cannabis breeders and researchers to maintain desirable germplasm for future use in breeding or for biotechnological and gene editing applications. Here, we report a simple and efficient cryopreservation method for long-term storage of <i>Ca...

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Bibliographic Details
Main Authors: Daniel Gaudet, Narendra Singh Yadav, Aleksei Sorokin, Andriy Bilichak, Igor Kovalchuk
Format: Article
Language:English
Published: MDPI AG 2020-05-01
Series:Plants
Subjects:
Cannabis sativa
pollen germination assay
cryopreservation
long-term storage
DAPI staining of germinated pollen
vegetative nuclei
Online Access:https://www.mdpi.com/2223-7747/9/5/665

Internet

https://www.mdpi.com/2223-7747/9/5/665

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