Rational design and structure-based engineering of alkaline pectate lyase from Paenibacillus sp. 0602 to improve thermostability

Rational design and structure-based engineering of alkaline pectate lyase from Paenibacillus sp. 0602 to improve thermostability

Abstract Background Ramie degumming is often carried out at high temperatures; therefore, thermostable alkaline pectate lyase (PL) is beneficial for ramie degumming for industrial applications. Thermostable PLs are usually obtained by exploring new enzymes or reconstructing existing enzyme by ration...

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Bibliographic Details
Main Authors: Zhanping Zhou, Xiao Wang
Format: Article
Language:English
Published: BMC 2021-05-01
Series:BMC Biotechnology
Subjects:
Protein engineering
Alkaline pectate lyase
Thermostability
PoPMuSiC
Site-directed mutagenesis
Online Access:https://doi.org/10.1186/s12896-021-00693-8

Internet

https://doi.org/10.1186/s12896-021-00693-8

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