Interleukin-38 ameliorates poly(I:C) induced lung inflammation: therapeutic implications in respiratory viral infections
Abstract Interleukin-38 has recently been shown to have anti-inflammatory properties in lung inflammatory diseases. However, the effects of IL-38 in viral pneumonia remains unknown. In the present study, we demonstrate that circulating IL-38 concentrations together with IL-36α increased significantl...
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doaj-33444cd18c014f60b41511efa62b8b482021-01-10T12:07:01ZengNature Publishing GroupCell Death and Disease2041-48892021-01-0112111810.1038/s41419-020-03283-2Interleukin-38 ameliorates poly(I:C) induced lung inflammation: therapeutic implications in respiratory viral infectionsXun Gao0Paul Kay Sheung Chan1Grace Chung Yan Lui2David Shu Cheong Hui3Ida Miu-Ting Chu4Xiaoyu Sun5Miranda Sin-Man Tsang6Ben Chung Lap Chan7Christopher Wai-Kei Lam8Chun-Kwok Wong9Department of Chemical Pathology, The Chinese University of Hong KongDepartment of Microbiology, The Chinese University of Hong KongStanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong KongStanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong KongDepartment of Chemical Pathology, The Chinese University of Hong KongDepartment of Chemical Pathology, The Chinese University of Hong KongDepartment of Chemical Pathology, The Chinese University of Hong KongInstitute of Chinese Medicine and State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants, The Chinese University of Hong KongFaculty of Medicine and State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and TechnologyDepartment of Chemical Pathology, The Chinese University of Hong KongAbstract Interleukin-38 has recently been shown to have anti-inflammatory properties in lung inflammatory diseases. However, the effects of IL-38 in viral pneumonia remains unknown. In the present study, we demonstrate that circulating IL-38 concentrations together with IL-36α increased significantly in influenza and COVID-19 patients, and the level of IL-38 and IL-36α correlated negatively and positively with disease severity and inflammation, respectively. In the co-cultured human respiratory epithelial cells with macrophages to mimic lung microenvironment in vitro, IL-38 was able to alleviate inflammatory responses by inhibiting poly(I:C)-induced overproduction of pro-inflammatory cytokines and chemokines through intracellular STAT1, STAT3, p38 MAPK, ERK1/2, MEK, and NF-κB signaling pathways. Intriguingly, transcriptomic profiling revealed that IL-38 targeted genes were associated with the host innate immune response to virus. We also found that IL-38 counteracts the biological processes induced by IL-36α in the co-culture. Furthermore, the administration of recombinant IL-38 could mitigate poly I:C-induced lung injury, with reduced early accumulation of neutrophils and macrophages in bronchoalveolar lavage fluid, activation of lymphocytes, production of pro-inflammatory cytokines and chemokines and permeability of the alveolar-epithelial barrier. Taken together, our study indicates that IL-38 plays a crucial role in protection from exaggerated pulmonary inflammation during poly(I:C)-induced pneumonia, thereby providing the basis of a novel therapeutic target for respiratory viral infections.https://doi.org/10.1038/s41419-020-03283-2 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xun Gao Paul Kay Sheung Chan Grace Chung Yan Lui David Shu Cheong Hui Ida Miu-Ting Chu Xiaoyu Sun Miranda Sin-Man Tsang Ben Chung Lap Chan Christopher Wai-Kei Lam Chun-Kwok Wong |
spellingShingle |
Xun Gao Paul Kay Sheung Chan Grace Chung Yan Lui David Shu Cheong Hui Ida Miu-Ting Chu Xiaoyu Sun Miranda Sin-Man Tsang Ben Chung Lap Chan Christopher Wai-Kei Lam Chun-Kwok Wong Interleukin-38 ameliorates poly(I:C) induced lung inflammation: therapeutic implications in respiratory viral infections Cell Death and Disease |
author_facet |
Xun Gao Paul Kay Sheung Chan Grace Chung Yan Lui David Shu Cheong Hui Ida Miu-Ting Chu Xiaoyu Sun Miranda Sin-Man Tsang Ben Chung Lap Chan Christopher Wai-Kei Lam Chun-Kwok Wong |
author_sort |
Xun Gao |
title |
Interleukin-38 ameliorates poly(I:C) induced lung inflammation: therapeutic implications in respiratory viral infections |
title_short |
Interleukin-38 ameliorates poly(I:C) induced lung inflammation: therapeutic implications in respiratory viral infections |
title_full |
Interleukin-38 ameliorates poly(I:C) induced lung inflammation: therapeutic implications in respiratory viral infections |
title_fullStr |
Interleukin-38 ameliorates poly(I:C) induced lung inflammation: therapeutic implications in respiratory viral infections |
title_full_unstemmed |
Interleukin-38 ameliorates poly(I:C) induced lung inflammation: therapeutic implications in respiratory viral infections |
title_sort |
interleukin-38 ameliorates poly(i:c) induced lung inflammation: therapeutic implications in respiratory viral infections |
publisher |
Nature Publishing Group |
series |
Cell Death and Disease |
issn |
2041-4889 |
publishDate |
2021-01-01 |
description |
Abstract Interleukin-38 has recently been shown to have anti-inflammatory properties in lung inflammatory diseases. However, the effects of IL-38 in viral pneumonia remains unknown. In the present study, we demonstrate that circulating IL-38 concentrations together with IL-36α increased significantly in influenza and COVID-19 patients, and the level of IL-38 and IL-36α correlated negatively and positively with disease severity and inflammation, respectively. In the co-cultured human respiratory epithelial cells with macrophages to mimic lung microenvironment in vitro, IL-38 was able to alleviate inflammatory responses by inhibiting poly(I:C)-induced overproduction of pro-inflammatory cytokines and chemokines through intracellular STAT1, STAT3, p38 MAPK, ERK1/2, MEK, and NF-κB signaling pathways. Intriguingly, transcriptomic profiling revealed that IL-38 targeted genes were associated with the host innate immune response to virus. We also found that IL-38 counteracts the biological processes induced by IL-36α in the co-culture. Furthermore, the administration of recombinant IL-38 could mitigate poly I:C-induced lung injury, with reduced early accumulation of neutrophils and macrophages in bronchoalveolar lavage fluid, activation of lymphocytes, production of pro-inflammatory cytokines and chemokines and permeability of the alveolar-epithelial barrier. Taken together, our study indicates that IL-38 plays a crucial role in protection from exaggerated pulmonary inflammation during poly(I:C)-induced pneumonia, thereby providing the basis of a novel therapeutic target for respiratory viral infections. |
url |
https://doi.org/10.1038/s41419-020-03283-2 |
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