Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris

Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris

Abstract Background The microbial transglutaminase (MTG) is inactive when only the mature sequence is expressed in Pichia pastoris. Although co-expression of MTG and its N-terminal pro-peptide can obtain the active MTG, the enzyme activity was still low. One of the basic steps for strain improvement...

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Bibliographic Details
Main Authors: Xiaoping Song, Changsheng Shao, Yugang Guo, Yajie Wang, Jingjing Cai
Format: Article
Language:English
Published: BMC 2019-07-01
Series:BMC Biotechnology
Subjects:
Transglutaminase
Pichia pastoris
rDNA
Pro-peptide
Co-expression
Online Access:http://link.springer.com/article/10.1186/s12896-019-0542-6

Internet

http://link.springer.com/article/10.1186/s12896-019-0542-6

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