Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris
Abstract Background The microbial transglutaminase (MTG) is inactive when only the mature sequence is expressed in Pichia pastoris. Although co-expression of MTG and its N-terminal pro-peptide can obtain the active MTG, the enzyme activity was still low. One of the basic steps for strain improvement...
Main Authors: | Xiaoping Song, Changsheng Shao, Yugang Guo, Yajie Wang, Jingjing Cai |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2019-07-01
|
Series: | BMC Biotechnology |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12896-019-0542-6 |
Similar Items
-
Effect of Recombinant Transglutaminase on the Quality Characteristics of Cooked Beef Meatballs
by: Fatma ERSÖZ, et al.
Published: (2021-03-01) -
Comparison of zeocin and G418 resistance markers in Pichia pastoris
by: Moy, Allison
Published: (2009) -
ProduÃÃo heterÃloga de frutalina em Pichia pastoris
by: Wagner Pereira Felix
Published: (2008) -
Cloning and expression of xylanase variants in Pichia pastoris
by: Govindarajulu, Natasha
Published: (2017) -
A Pichia pastoris fermentation process to express Mucor pusillus rennin
by: Toan Hong Phuoc, et al.