Spindle-F Is the Central Mediator of Ik2 Kinase-Dependent Dendrite Pruning in Drosophila Sensory Neurons.
During development, certain Drosophila sensory neurons undergo dendrite pruning that selectively eliminates their dendrites but leaves the axons intact. How these neurons regulate pruning activity in the dendrites remains unknown. Here, we identify a coiled-coil protein Spindle-F (Spn-F) that is req...
Main Authors: | , , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015-11-01
|
Series: | PLoS Genetics |
Online Access: | http://europepmc.org/articles/PMC4634852?pdf=render |
id |
doaj-344adb91182c4431b3e2efac028e93aa |
---|---|
record_format |
Article |
spelling |
doaj-344adb91182c4431b3e2efac028e93aa2020-11-24T21:41:37ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042015-11-011111e100564210.1371/journal.pgen.1005642Spindle-F Is the Central Mediator of Ik2 Kinase-Dependent Dendrite Pruning in Drosophila Sensory Neurons.Tzu LinPo-Yuan PanYu-Ting LaiKai-Wen ChiangHsin-Lun HsiehYi-Ping WuJian-Ming KeMyong-Chol LeeShih-Sian LiaoHsueh-Tzu ShihChiou-Yang TangShi-Bing YangHsu-Chen ChengJune-Tai WuYuh-Nung JanHsiu-Hsiang LeeDuring development, certain Drosophila sensory neurons undergo dendrite pruning that selectively eliminates their dendrites but leaves the axons intact. How these neurons regulate pruning activity in the dendrites remains unknown. Here, we identify a coiled-coil protein Spindle-F (Spn-F) that is required for dendrite pruning in Drosophila sensory neurons. Spn-F acts downstream of IKK-related kinase Ik2 in the same pathway for dendrite pruning. Spn-F exhibits a punctate pattern in larval neurons, whereas these Spn-F puncta become redistributed in pupal neurons, a step that is essential for dendrite pruning. The redistribution of Spn-F from puncta in pupal neurons requires the phosphorylation of Spn-F by Ik2 kinase to decrease Spn-F self-association, and depends on the function of microtubule motor dynein complex. Spn-F is a key component to link Ik2 kinase to dynein motor complex, and the formation of Ik2/Spn-F/dynein complex is critical for Spn-F redistribution and for dendrite pruning. Our findings reveal a novel regulatory mechanism for dendrite pruning achieved by temporal activation of Ik2 kinase and dynein-mediated redistribution of Ik2/Spn-F complex in neurons.http://europepmc.org/articles/PMC4634852?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tzu Lin Po-Yuan Pan Yu-Ting Lai Kai-Wen Chiang Hsin-Lun Hsieh Yi-Ping Wu Jian-Ming Ke Myong-Chol Lee Shih-Sian Liao Hsueh-Tzu Shih Chiou-Yang Tang Shi-Bing Yang Hsu-Chen Cheng June-Tai Wu Yuh-Nung Jan Hsiu-Hsiang Lee |
spellingShingle |
Tzu Lin Po-Yuan Pan Yu-Ting Lai Kai-Wen Chiang Hsin-Lun Hsieh Yi-Ping Wu Jian-Ming Ke Myong-Chol Lee Shih-Sian Liao Hsueh-Tzu Shih Chiou-Yang Tang Shi-Bing Yang Hsu-Chen Cheng June-Tai Wu Yuh-Nung Jan Hsiu-Hsiang Lee Spindle-F Is the Central Mediator of Ik2 Kinase-Dependent Dendrite Pruning in Drosophila Sensory Neurons. PLoS Genetics |
author_facet |
Tzu Lin Po-Yuan Pan Yu-Ting Lai Kai-Wen Chiang Hsin-Lun Hsieh Yi-Ping Wu Jian-Ming Ke Myong-Chol Lee Shih-Sian Liao Hsueh-Tzu Shih Chiou-Yang Tang Shi-Bing Yang Hsu-Chen Cheng June-Tai Wu Yuh-Nung Jan Hsiu-Hsiang Lee |
author_sort |
Tzu Lin |
title |
Spindle-F Is the Central Mediator of Ik2 Kinase-Dependent Dendrite Pruning in Drosophila Sensory Neurons. |
title_short |
Spindle-F Is the Central Mediator of Ik2 Kinase-Dependent Dendrite Pruning in Drosophila Sensory Neurons. |
title_full |
Spindle-F Is the Central Mediator of Ik2 Kinase-Dependent Dendrite Pruning in Drosophila Sensory Neurons. |
title_fullStr |
Spindle-F Is the Central Mediator of Ik2 Kinase-Dependent Dendrite Pruning in Drosophila Sensory Neurons. |
title_full_unstemmed |
Spindle-F Is the Central Mediator of Ik2 Kinase-Dependent Dendrite Pruning in Drosophila Sensory Neurons. |
title_sort |
spindle-f is the central mediator of ik2 kinase-dependent dendrite pruning in drosophila sensory neurons. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Genetics |
issn |
1553-7390 1553-7404 |
publishDate |
2015-11-01 |
description |
During development, certain Drosophila sensory neurons undergo dendrite pruning that selectively eliminates their dendrites but leaves the axons intact. How these neurons regulate pruning activity in the dendrites remains unknown. Here, we identify a coiled-coil protein Spindle-F (Spn-F) that is required for dendrite pruning in Drosophila sensory neurons. Spn-F acts downstream of IKK-related kinase Ik2 in the same pathway for dendrite pruning. Spn-F exhibits a punctate pattern in larval neurons, whereas these Spn-F puncta become redistributed in pupal neurons, a step that is essential for dendrite pruning. The redistribution of Spn-F from puncta in pupal neurons requires the phosphorylation of Spn-F by Ik2 kinase to decrease Spn-F self-association, and depends on the function of microtubule motor dynein complex. Spn-F is a key component to link Ik2 kinase to dynein motor complex, and the formation of Ik2/Spn-F/dynein complex is critical for Spn-F redistribution and for dendrite pruning. Our findings reveal a novel regulatory mechanism for dendrite pruning achieved by temporal activation of Ik2 kinase and dynein-mediated redistribution of Ik2/Spn-F complex in neurons. |
url |
http://europepmc.org/articles/PMC4634852?pdf=render |
work_keys_str_mv |
AT tzulin spindlefisthecentralmediatorofik2kinasedependentdendritepruningindrosophilasensoryneurons AT poyuanpan spindlefisthecentralmediatorofik2kinasedependentdendritepruningindrosophilasensoryneurons AT yutinglai spindlefisthecentralmediatorofik2kinasedependentdendritepruningindrosophilasensoryneurons AT kaiwenchiang spindlefisthecentralmediatorofik2kinasedependentdendritepruningindrosophilasensoryneurons AT hsinlunhsieh spindlefisthecentralmediatorofik2kinasedependentdendritepruningindrosophilasensoryneurons AT yipingwu spindlefisthecentralmediatorofik2kinasedependentdendritepruningindrosophilasensoryneurons AT jianmingke spindlefisthecentralmediatorofik2kinasedependentdendritepruningindrosophilasensoryneurons AT myongchollee spindlefisthecentralmediatorofik2kinasedependentdendritepruningindrosophilasensoryneurons AT shihsianliao spindlefisthecentralmediatorofik2kinasedependentdendritepruningindrosophilasensoryneurons AT hsuehtzushih spindlefisthecentralmediatorofik2kinasedependentdendritepruningindrosophilasensoryneurons AT chiouyangtang spindlefisthecentralmediatorofik2kinasedependentdendritepruningindrosophilasensoryneurons AT shibingyang spindlefisthecentralmediatorofik2kinasedependentdendritepruningindrosophilasensoryneurons AT hsuchencheng spindlefisthecentralmediatorofik2kinasedependentdendritepruningindrosophilasensoryneurons AT junetaiwu spindlefisthecentralmediatorofik2kinasedependentdendritepruningindrosophilasensoryneurons AT yuhnungjan spindlefisthecentralmediatorofik2kinasedependentdendritepruningindrosophilasensoryneurons AT hsiuhsianglee spindlefisthecentralmediatorofik2kinasedependentdendritepruningindrosophilasensoryneurons |
_version_ |
1725921029592711168 |