Efficient Incorporation of Unnatural Amino Acids into Proteins with a Robust Cell-Free System

Unnatural proteins are crucial biomacromolecules and have been widely applied in fundamental science, novel biopolymer materials, enzymes, and therapeutics. Cell-free protein synthesis (CFPS) system can serve as a robust platform to synthesize unnatural proteins by highly effective site-specific inc...

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Bibliographic Details
Main Authors: Wei Gao, Ning Bu, Yuan Lu
Format: Article
Language:English
Published: MDPI AG 2019-02-01
Series:Methods and Protocols
Subjects:
Online Access:https://www.mdpi.com/2409-9279/2/1/16