Effect of cooling to different sub-zero temperatures on boar sperm cryosurvival
Objective: To compare different cooling temperatures before ice formation on pig sperm quality, before and after cryopreservation. Methods: Semen diluted in BF5 was cooled from 23 °C to 5 °C (1% glycerol, 200 × 106 cells/mL). Sperm were packaged in plastic straws, and maintained at +5 °C per 16 h. 1...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2016-03-01
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Series: | Asian Pacific Journal of Reproduction |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2305050015000615 |