Effect of cooling to different sub-zero temperatures on boar sperm cryosurvival

Objective: To compare different cooling temperatures before ice formation on pig sperm quality, before and after cryopreservation. Methods: Semen diluted in BF5 was cooled from 23 °C to 5 °C (1% glycerol, 200 × 106 cells/mL). Sperm were packaged in plastic straws, and maintained at +5 °C per 16 h. 1...

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Bibliographic Details
Main Authors: Angelica Garcia-Olivares, Cesar Garzon-Perez, Oscar Gutierrez-Perez, Alfredo Medrano
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2016-03-01
Series:Asian Pacific Journal of Reproduction
Subjects:
Pig
Online Access:http://www.sciencedirect.com/science/article/pii/S2305050015000615