Analytical performance of a multiplex Real-Time PCR assay using TaqMan probes for quantification of Trypanosoma cruzi satellite DNA in blood samples.

Analytical performance of a multiplex Real-Time PCR assay using TaqMan probes for quantification of Trypanosoma cruzi satellite DNA in blood samples.

BACKGROUND: The analytical validation of sensitive, accurate and standardized Real-Time PCR methods for Trypanosoma cruzi quantification is crucial to provide a reliable laboratory tool for diagnosis of recent infections as well as for monitoring treatment efficacy. METHODS/PRINCIPAL FINDINGS: We ha...

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Bibliographic Details
Main Authors: Tomas Duffy, Carolina I Cura, Juan C Ramirez, Teresa Abate, Nelly M Cayo, Rudy Parrado, Zoraida Diaz Bello, Elsa Velazquez, Arturo Muñoz-Calderon, Natalia A Juiz, Joaquín Basile, Lineth Garcia, Adelina Riarte, Julio R Nasser, Susana B Ocampo, Zaida E Yadon, Faustino Torrico, Belkisyole Alarcón de Noya, Isabela Ribeiro, Alejandro G Schijman
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS Neglected Tropical Diseases
Online Access:http://europepmc.org/articles/PMC3547845?pdf=render

Internet

http://europepmc.org/articles/PMC3547845?pdf=render

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