Single-cell transcriptome conservation in a comparative analysis of fresh and cryopreserved human skin tissue: pilot in localized scleroderma

Single-cell transcriptome conservation in a comparative analysis of fresh and cryopreserved human skin tissue: pilot in localized scleroderma

Abstract Background The purpose of this study was to assess variability in cell composition and cell-specific gene expression in the skin of patients with localized scleroderma (LS) utilizing CryoStor® CS10 in comparison to RPMI to produce adequate preservation of tissue samples and cell types of in...

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Bibliographic Details
Main Authors: Emily Mirizio, Tracy Tabib, Xiao Wang, Wei Chen, Christopher Liu, Robert Lafyatis, Heidi Jacobe, Kathryn S. Torok
Format: Article
Language:English
Published: BMC 2020-11-01
Series:Arthritis Research & Therapy
Subjects:
Localized scleroderma
Morphea
Single-cell RNA sequencing
Pediatric rheumatology
Cryopreservation
Transcriptome expression
Online Access:http://link.springer.com/article/10.1186/s13075-020-02343-4

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http://link.springer.com/article/10.1186/s13075-020-02343-4

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