Quantitative real-time analysis of the efflux by the MacAB-TolC tripartite efflux pump clarifies the role of ATP hydrolysis within mechanotransmission mechanism

Quantitative real-time analysis of the efflux by the MacAB-TolC tripartite efflux pump clarifies the role of ATP hydrolysis within mechanotransmission mechanism

Souabni et al. introduce a new analytical method for the study of ABC tripartite efflux pumps, by using a lipid scaffold that mimics the protein’s native environment and a spectroscopic assay, employing quantum dots (QD), that monitors ATP hydrolysis and substrate transport in real-time. When applie...

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Bibliographic Details
Main Authors: Hager Souabni, William Batista dos Santos, Quentin Cece, Laurent J. Catoire, Dhenesh Puvanendran, Vassiliy N. Bavro, Martin Picard
Format: Article
Language:English
Published: Nature Publishing Group 2021-04-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-021-01997-3

Internet

https://doi.org/10.1038/s42003-021-01997-3

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