Expansion microscopy-based imaging of nuclear structures in cultured cells

Summary: Expansion microscopy is a sample preparation technique in which fixed and immunostained cells or tissues are embedded in a cross-linked network of swellable polyelectrolyte hydrogel that expands isotropically upon addition of deionized water. We utilize the X10 method for tenfold expansion...

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Bibliographic Details
Main Authors: Antoine Gaudreau-Lapierre, Kirk Mulatz, Jean-Claude Béïque, Laura Trinkle-Mulcahy
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003373