One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR).

One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR).

Novel applications based on the bacterial CRISPR system make genetic, genomic, transcriptional and epigenomic engineering widely accessible for the first time. A significant advantage of CRISPR over previous methods is its tremendous adaptability due to its bipartite nature. Cas9 or its engineered v...

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Bibliographic Details
Main Authors: Christopher T Breunig, Tamara Durovic, Andrea M Neuner, Valentin Baumann, Maximilian F Wiesbeck, Anna Köferle, Magdalena Götz, Jovica Ninkovic, Stefan H Stricker
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5922533?pdf=render

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http://europepmc.org/articles/PMC5922533?pdf=render

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