Characterization of the Castanopsis carlesii Deadwood Mycobiome by Pacbio Sequencing of the Full-Length Fungal Nuclear Ribosomal Internal Transcribed Spacer (ITS)

Characterization of the Castanopsis carlesii Deadwood Mycobiome by Pacbio Sequencing of the Full-Length Fungal Nuclear Ribosomal Internal Transcribed Spacer (ITS)

Short-read next generation sequencing (NGS) platforms can easily and quickly generate thousands to hundreds of thousands of sequences per sample. However, the limited length of these sequences can cause problems during fungal taxonomic identification. Here we validate the use of Pacbio sequencing, a...

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Bibliographic Details
Main Authors: Witoon Purahong, Ausana Mapook, Yu-Ting Wu, Chaur-Tzuhn Chen
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-05-01
Series:Frontiers in Microbiology
Subjects:
NGS
long-read sequencing
fungal community
wood-inhabiting fungi
fungal diversity
species identification
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.00983/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2019.00983/full

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