Type I Interferon Transcriptional Signature in Neutrophils and Low-Density Granulocytes Are Associated with Tissue Damage in Malaria
Neutrophils are the most abundant leukocyte population in the bloodstream, the primary compartment of Plasmodium sp. infection. However, the role of these polymorphonuclear cells in mediating either the resistance or the pathogenesis of malaria is poorly understood. We report that circulating neutro...
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doaj-3516ce503d8b471ba3586c940a1da1ed2020-11-24T21:11:29ZengElsevierCell Reports2211-12472015-12-0113122829284110.1016/j.celrep.2015.11.055Type I Interferon Transcriptional Signature in Neutrophils and Low-Density Granulocytes Are Associated with Tissue Damage in MalariaBruno Coelho Rocha0Pedro Elias Marques1Fabiana Maria de Souza Leoratti2Caroline Junqueira3Dhelio Batista Pereira4Lis Ribeiro do Valle Antonelli5Gustavo Batista Menezes6Douglas Taylor Golenbock7Ricardo Tostes Gazzinelli8Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, BrazilDepartamento de Morfologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, BrazilCentro de Pesquisas Rene Rachou, Fundação Oswaldo Cruz–Minas Gerais, Belo Horizonte 30190-002, BrazilCentro de Pesquisas Rene Rachou, Fundação Oswaldo Cruz–Minas Gerais, Belo Horizonte 30190-002, BrazilCentro de Pesquisas em Medicina Tropical, Fundação Oswaldo Cruz–Rondônia, Porto Velho 76812-329, BrazilCentro de Pesquisas Rene Rachou, Fundação Oswaldo Cruz–Minas Gerais, Belo Horizonte 30190-002, BrazilDepartamento de Morfologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, BrazilDepartment of Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USADepartamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, BrazilNeutrophils are the most abundant leukocyte population in the bloodstream, the primary compartment of Plasmodium sp. infection. However, the role of these polymorphonuclear cells in mediating either the resistance or the pathogenesis of malaria is poorly understood. We report that circulating neutrophils from malaria patients are highly activated, as indicated by a strong type I interferon transcriptional signature, increased expression of surface activation markers, enhanced release of reactive oxygen species and myeloperoxidase, and a high frequency of low-density granulocytes. The activation of neutrophils was associated with increased levels of serum alanine and aspartate aminotransferases, indicating liver damage. In a rodent malaria model, we observed intense recruitment of neutrophils to liver sinusoids. Neutrophil migration and IL-1β and chemokine expression as well as liver damage were all dependent on type I interferon signaling. The data suggest that type I interferon signaling has a central role in neutrophil activation and malaria pathogenesis.http://www.sciencedirect.com/science/article/pii/S2211124715014047 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bruno Coelho Rocha Pedro Elias Marques Fabiana Maria de Souza Leoratti Caroline Junqueira Dhelio Batista Pereira Lis Ribeiro do Valle Antonelli Gustavo Batista Menezes Douglas Taylor Golenbock Ricardo Tostes Gazzinelli |
spellingShingle |
Bruno Coelho Rocha Pedro Elias Marques Fabiana Maria de Souza Leoratti Caroline Junqueira Dhelio Batista Pereira Lis Ribeiro do Valle Antonelli Gustavo Batista Menezes Douglas Taylor Golenbock Ricardo Tostes Gazzinelli Type I Interferon Transcriptional Signature in Neutrophils and Low-Density Granulocytes Are Associated with Tissue Damage in Malaria Cell Reports |
author_facet |
Bruno Coelho Rocha Pedro Elias Marques Fabiana Maria de Souza Leoratti Caroline Junqueira Dhelio Batista Pereira Lis Ribeiro do Valle Antonelli Gustavo Batista Menezes Douglas Taylor Golenbock Ricardo Tostes Gazzinelli |
author_sort |
Bruno Coelho Rocha |
title |
Type I Interferon Transcriptional Signature in Neutrophils and Low-Density Granulocytes Are Associated with Tissue Damage in Malaria |
title_short |
Type I Interferon Transcriptional Signature in Neutrophils and Low-Density Granulocytes Are Associated with Tissue Damage in Malaria |
title_full |
Type I Interferon Transcriptional Signature in Neutrophils and Low-Density Granulocytes Are Associated with Tissue Damage in Malaria |
title_fullStr |
Type I Interferon Transcriptional Signature in Neutrophils and Low-Density Granulocytes Are Associated with Tissue Damage in Malaria |
title_full_unstemmed |
Type I Interferon Transcriptional Signature in Neutrophils and Low-Density Granulocytes Are Associated with Tissue Damage in Malaria |
title_sort |
type i interferon transcriptional signature in neutrophils and low-density granulocytes are associated with tissue damage in malaria |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2015-12-01 |
description |
Neutrophils are the most abundant leukocyte population in the bloodstream, the primary compartment of Plasmodium sp. infection. However, the role of these polymorphonuclear cells in mediating either the resistance or the pathogenesis of malaria is poorly understood. We report that circulating neutrophils from malaria patients are highly activated, as indicated by a strong type I interferon transcriptional signature, increased expression of surface activation markers, enhanced release of reactive oxygen species and myeloperoxidase, and a high frequency of low-density granulocytes. The activation of neutrophils was associated with increased levels of serum alanine and aspartate aminotransferases, indicating liver damage. In a rodent malaria model, we observed intense recruitment of neutrophils to liver sinusoids. Neutrophil migration and IL-1β and chemokine expression as well as liver damage were all dependent on type I interferon signaling. The data suggest that type I interferon signaling has a central role in neutrophil activation and malaria pathogenesis. |
url |
http://www.sciencedirect.com/science/article/pii/S2211124715014047 |
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